Take advantage of our wide range of established and validated dermal pharmacology and efficacy studies in any species. We have both normal and diseased research models with single, multiple or infusion/slow release dose administration of small and large molecules, stem cells, metals, nanoparticles or devices. Our team offers custom studies and model development.

Comparative Bioscience’s Wound Healing Models and Capabilities

Methods of Assessment

  • Dermal optical coherence tomography (OCT)
  • Enzyme-linked immune absorbent assays
  • Immunohistochemistry assays
  • Laser Doppler for blood flow
  • Silhouette photography
Delayed Type Hypersensitivity (DTH) Studies

Delayed-type hypersensitivity, sensitization or maximization studies may be employed to determine if the test article is a dermal sensitizer and if it can suppress an induced DTH reaction. CBI offers expertise in the following DTH studies:

  • Skin sensitization (maximization) study using the Beuhler or Magnusson-Kligman method
  • Delayed type hypersensitivity study:
    • Oxazolone (TH-2 mediated; back skin or ear thickness)
    • TH-1 mediated hapten-induced: DNFB, DNCB, TNFB, TNCB, TMA, etc.
    • Back skin or ear thickness
  • Intradermal methylated BSA
  • Murine local lymph node assay for assessment of allergic contact dermatitis potential
  • Passive cutaneous anaphylaxis
  • Chemical irritant dermatitis
Delivery Devices

Dermal delivery devices are important in the delivery of drugs for chronic pain and chronic metabolic diseases — any condition in which slow continuous systemic administration of drugs is needed. The local or topical effects of these devices on the skin, as well as the ability of the device to deliver the test article, may be determined through toxicologic, pharmacologic or pharmacokinetic assessment.

Dermal Inflammation

In these protocol-driven studies, acute or chronic local dermal inflammation is induced by a variety of means. Test articles are administered and the local anti-inflammatory activity is assessed in a variety of research models. Studies include acute dermal inflammation studies and chronic dermal inflammation studies.

Studies include:

  • Dermal sensitization (Beuhler, Magnusson-Kligman methods)
  • Imiquimod-induced murine model of psoriasis
Wound Healing

Wound healing studies may be conducted in normal or diabetic (streptozotocin-induced) models. Uniform wounds of full thickness, split thickness or single cuts are created surgically and healing is assessed. The test article is administered and effects of test article on size, rate and character of healing are determined. Clinical signs, OCT, laser Doppler, histopathology, immunohistochemistry, histomorphometry/digital image analysis and tensile strength are typically used to assess wound healing.

Dermal Burns

A first or second degree burn is induced in a dermal burn research model by thermal or ultraviolet light methods and the effects of the test article on healing and scar formation are assessed. The effects of the test article on burn size and healing are evaluated using clinical signs, bacteriology, histopathology, special stains such as sirius red and trichrome, immunohistochemistry, digital image analysis, and in vitro assays such as Sircol®. We also offer sophisticated assessments including OCT, laser Doppler for blood flow, silhouette photography and enzyme-linked immune absorbent assays.

View an overview of our Thermal Burn Modeling services.

Dermal Fibrosis (Scleroderma)

In this model, dermal fibrosis (also called scleroderma) is induced by bleomycin administration. The effects of the test article on fibrosis formation are evaluated by assessing clinical signs, histopathology, special stains such as sirius red and trichrome, immunohistochemistry assays, digital image analysis and in vitro assays such as Sircol®.

Dermal Infection
Dermal Scarring

We use well-characterized models for scar and keloid formation. The effects of the test article on scar formation are assessed using clinical signs, histopathology, special stains such as sirius red and trichrome, immunohistochemistry, digital image analysis and in vitro assays such as Sircol®.