Cardiovascular and Pulmonary Pharmacology

Comparative Biosciences has extensive experience in cardiac, pulmonary and vascular pharmacology modeling, including atherosclerosis, pulmonary hypertension, LAD model and balloon angioplasty with stents. We are happy to provide detailed study designs and validation data upon request. CBI offers a variety of models, and we can also design custom models to meet your needs.

Importantly, CBI prepares and provides state-of-the-art-studies with high quality comprehensive reports that are suitable for regulatory submission.

Preclinical cardiovascular and pulmonary models and capabilities

Cardiac Models

  • Myocardial Ischemia and Reperfusion (LAD)
  • Doxarubicin myocyte injury (due to platinum chemotherapeutics)
  • Regenerative cardiovascular medicine and surgical transplantation
  • Models of Atherosclerosis
  • Myocardial infarction – check out our presentation
  • Chronic total occlusion (CTO) creation

Pulmonary Models

  • Hyperoxia injury model 
  • Monocrotaline-induced Pulmonary Hypertension – check out our presentation
  • Bleomycin-induced Pulmonary Fibrosis – check out our presentation 
  • Pulmonary Arterial Hypertension
  • Endotracheal long term anesthesia
  • Custom model development

Vascular Models

  • Models of Atherosclerosis
  • Cerebral stroke (middle cerebral artery (MCA) occlusion)
  • Arterio-venous (AV) fistulas and grafts / dialysis models
  • Bleeding, hemostasis and blood loss-splenic and hepatic laceration
  • IVC/CVC and other vascular device testing
  • Balloon Angioplasty and Restenosis, Including Stents
  • In-stent restenosis (ISR)
  • Hypertension
  • Custom model development

Methods of Assessment

  • Arterio-venous (AV) fistulas and grafts / dialysis models
  • Bleeding, hemostasis and blood loss
  • Hypertension
    • Monocrotaline-induced pulmonary hypertension in rats
  • Hyperoxia injury model
  • Ischemia and reperfusion models
    • Cerebral stroke (middle cerebral artery (MCA) occlusion)
    • Other organ surgical occlusions
  • IVC/CVC and other vascular device testing
  • Pulmonary injury
  • Regenerative cardiovascular medicine and surgical transplantation

And with our collaborators at BioSurg, Sacramento, a cardiovascular CRO that specialized in cardiovascular procedures including open heart and open chest surgery and cardiac bipass in large animals:

  • Aneurysms (elastase derived, surgical bifurcate/sidewall/terminal/graft)
  • Chronic total occlusion (CTO) creation (surgical and interventional)
  • Coronary artery bypass grafts
  • Drug deposition
  • Endothelialization
  • In-stent restenosis (ISR)
  • Myocardial infarct (MI) models (surgical and interventional)
  • Rete mirabile (AV malformation)
  • Thrombosis/clot, deep vein thrombosis (DVT)

Cardiovascular Models

Monocrotaline-induced Pulmonary Hypertension
1 Monocrotaline: Smooth muscle hypertrophy, perivascular edema and pulmonary inflammation are present. Masson’s trichrome.
Bleomycin-induced Pulmonary Fibrosis
2 Bleomycin lung: Interstitial fibrosis, vascular edema and pulmonary inflammation are present. Masson’s trichrome.
3 Bleomycin skin: Dermal fibrosis, vascular edema and inflammation are present. Masson’s trichrome.
Myocardial Ischemia and Reperfusion (LAD)
4 Ligation of LAD: Evan’s blue dye exclusion demonstrating area at risk.
7 NORMAL RAT HEART. MASSON’S TRICHROME.
6 LAD ligation: Moderate transmural thinning and fibrosis. Masson’s trichrome.
7 LAD ligation: Severe transmural thinning and fibrosis. Masson’s trichrome.
Balloon Angioplasty and Restenosis, Including Stents
8 Normal rabbit ear. Movat’s pentachrome.
9 Balloon angioplasty with restenosis injury rabbit ear. Movat’s pentachrome.
Models of Atherosclerosis
10 ApoE MOUSE, HIGH FAT DIET. THE AORTA IS OCCLUDED WITH CHOLESTEROL PLAQUES. MOVAT'S PENTACHROME.

Progression and regression studies in ApoE mice

Feeding a high fat diet to ApoE mice as well as rabbits, guinea pigs and swine results in hyperlipidemia, steatosis and atherosclerotic arterial changes.

  • Hyperlidemia in high-fat-fed Watanabe rabbits, normal rabbits and minipigs
  • Hyperlipidemia in high-fat-fed hamsters or guinea pigs
  • Mutant mouse models