CBI offers a number of special techniques in histology and also welcomes customized techniques.
Ocular Histopathology
CBI is skilled in the preparation of eyes from species ranging from laboratory animals to primates and humans. We offer paraffin, plastic and cryosections for routine, and special and immunohistochemical staining. Furthermore, we provide digital image analysis/morphometry for assessment of lesions such as subretinal plaque formation, retinal neovascular proliferation, ocular inflammation, retinal whole mount preparations, and corneal ulceration or thinning. Read more about our ocular studies.
Arthritis
CBI provides high-quality preparation of rodent joints from inflammation and arthritis models including decalcification, careful orientation of the joint, sectioning to include joint space, cartilage, synovium, and related soft tissues. Scoring of the cartilage changes, inflammation, pannus formation, synovial changes, and periarticular soft tissues are provided in a complete report with statistical analysis and photomicroscopy. Immunohistochemistry is also available. Read more about our athrititis and immune-mediated studies.
Atherosclerosis
CBI is experienced and skilled in the preparation of gelatin or nongelatin aortas (modified Paigen) and brachiocephalic arteries in rodent Atherosclerosis models, including special staining (ex. Movat’s Pentachrome, Oil Red O) and serial sectioning of the entire vessel with selection of specific levels for specific morphometry of the plaque and vessels. Complete reports with statistical analysis of the plaque areas and ratios are provided. Immunohistochemistry is also available. Read more about our atherosclerosis study models.
Infarction Models
CBI offers specialized preparation, staining and assessment of infarction models such as rat myocardical infarction (LAD), and MCOA (stroke). Scoring of infarct size, inflammation, and other relevant changes are provided in a complete report with statistical analysis and photomicroscopy. Immunohistochemistry and histomorphometry are also available.
Neuromuscular junction assessment
CBI offers specialized preparation, staining, and assessment of muscle and nerves, including immunohistochemical assessment of the neuromuscular endplates. This method is of particular interest for assessment of botulinum products in development. Immunohistochemistry is also available.
Dermal Wound Healing Assessment
CBI offers specialized preparation, staining, and assessment of dermal wound healing including careful orientation of the skin and subcutis, special stains, assessment and scoring of epithelial integrity, inflammation, collagen formation, and healing. Immunohistochemistry is also available. Read more about our dermatologic inflammation models.
Inflammatory Bowel or Ulcerative Colitis Assessment
CBI offers specialized preparation, orientation staining, and assessment of intestine in models of ulcerative or inflammatory bowel disease including careful orientation of the intestine, assessment and scoring of ulcer formation, mucosal integrity and inflammation, crypt hyperplasia, and lymphocyte changes. Immunohistochemistry and histomorphometry are also available.
Osteoporosis Assessment
CBI offers specialized preparation, orientation staining, and assessment of bone density in animal models of osteoporosis including careful orientation of the bone, assessment, measurements, and scoring of trabecular number, size, perimeter, and area with histomorphometric assessment. Special stains for bone, cartilage, fibrosis, and osteoid as well as Immunohistochemistry for cell types such as osteoblasts and osteoclasts may be performed. Complete reports with statistical analysis of comparative bone loss between treatment groups are provided.