CBI provides a range of acute, chronic, and immune-mediated dermal pharmacology models in small animals and pigs.
Dermatological in vivo modeling
Local irritation and tolerability
To determine if a test article is a local irritant, it is applied topically to intact or abraded skin, with or without coverage. The site is scored using a Draize-type score.
Inflammation- acute, chronic
Acute or chronic inflammation is induced and the ability of the test article to suppress the inflammation is determined.
Wound healing
In these models (pigs, rabbits, rat), uniform wounds are created surgically and healing assessed. Typical wounds would include full thickness, split thickness, and single cuts. The test article is administered and the effects of the test article on the rate and character of healing are determined.
Drug delivery devices used on the skin
There are many new experimental methods and devices used to deliver a test article to the skin for either local or systemic absorption. The effects of these devices on the skin as well as the ability of the device to deliver the test article may be determined.
Delayed Type Hypersensitization
Test articles may be assessed for their abilities to reduce inflammation or conversely to determine if they themselves act as sensitizers.
Dermal DTH
In this model, DTH is induced with the established sensitizers, oxalazone, or TNBF. There are minimally two inductions followed by a challenge and treatment with the test article. In mice, the ear is generally used, in other species such as rabbit, guinea pig, or pig, the back skin is used. Ears are measured for thickness and skin is scored using a Draize type score.
- Oxalazone-Induced DTH
- TNBF-Induced DTH
Guinea Pig Sensitization
GP sensitization or maximization assays determine if test articles themselves are sensitizers. The test article is applied topically during induction, followed by challenge. Application sites are scored using Draize’s test. Typically, there is a vehicle, 2-3 dose levels of test article and a positive control sensitizer. Types of methods include:
- Guinea Pig Sensitization: Beuhler Method
- Guinea Pig Sensitization: Masson Kligmann Method