Heptanol-Induced Corneal Wound Healing
In this model, which is useful to assess agents that either slow or enhance corneal wound healing, a uniform lesion is surgically induced by hepatanol. The rate of healing of the lesion is assessed by fluorescein corneal photography. Pachymetry and slit lamp examination is also performed. At the end of the study, histopathology may be performed.
A typical study design:
- Groups with at least 6 rabbits group recommended
- Test article administration either intraocularly or systemically
- Daily assessments of photography, pachymetry, and slit lamp assessment
- Histopathology on eye at end of study
Models of Dry Eye
Dry eye, whether immune-mediated or age related, is a significant unmet medical need. There are several standard models for assessment of dry eye therapy available at CBI.
Rabbit Conconavalin A-induced dry eye
In this standard model of immune-mediated dry eye, the lacrimal gland is injected with ConA. A self-limiting dry eye develops over about two weeks. A typical study design includes:
- Groups with at least 6 rabbits group recommended
- Con A injections on Day 0 and Day 3
- Test article administration either intraocularly or systemically
- Daily assessments of photography, Schirmer tear test, tear break-up test, pachymetry, and slit lamp assessment
- Histopathology on eye and adenexal structures
Mouse botulinum-induced dry eye
For more information, contact CBI
Mouse scopolomine -induced dry eye
For more information, contact CBI
Models of Inflammation
Acute Inflammation
- LPS-induced inflammation
Immune Mediated
- Melanin-associated antigen-induced inflammation
- Ovalbumin-induced inflammation
For more information, contact CBI
Models of metabolic retinopathy
Both the db./db mouse and streptozotin-treated rats develop a diabetic type retinopathy over time. For more information, contact CBI.