Comparative Biosciences, Inc. has extensive experience in cardiac, pulmonary and vascular pharmacology modeling, including atherosclerosis in mice and rabbits, pulmonary hypertension, LAD model in rodents, balloon angioplasty in rats and rabbits with stents. We are happy to provide detailed study designs and validation data upon request. Established models at CBI include the following models and we are also pleased to design custom models for our clients. Contact CBI for more information and validation data.
Monocrotaline: Smooth muscle hypertrophy, perivascular edema and pulmonary inflammation are present. Masson's trichrome
Monocrotaline-induced Pulmonary Hypertension
Monocrotaline is administered to mice or rats and over a period of weeks, hypertrophy of pulmonary arterial walls results in pulmonary hypertension and inflammation.
Bleomycin-induced Pulmonary Fibrosis
Bleomycin is administered to mice or rats and over a period of weeks, fibrosis of the pulmonary interstitium develops resulting in pulmonary fibrosis, hypertension and inflammation. Dermal fibrosis develops at dermal injection sites also.
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| Bleomycin Lung: Interstitial fibrosis, vascular edema and pulmonary inflammation are present. Masson's Trichrome. | Bleomycin Skin: Dermal fibrosis, vascular edema and inflammation are present. Masson's Trichrome. |
Myocardial Ischemia and Reperfusion (LAD in rats)
Ligation of the left anterior descending (LAD) coronary artery in mice or rats results in infarction of the left ventricle with thinning of the ventricular free wall and fibrosis.
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| Ligation of LAD: Evan's Blue dye exclusion demonstrating area at risk. | Normal rat heart. Masson's Trichrome. |
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| LAD Ligation: Moderate transmural thinning and fibrosis. Masson's Trichrome. | LAD Ligation: Severe transmural thinning and fibrosis. Masson's Trichrome |
Balloon Angioplasty and Restenosis in Rats and Rabbits, including Stents
Balloon angioplasty may be performed on a variety of arteries in rodents rabbits and dogs resulting in neointimal proliferation and restenosis injury.
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| Normal rabbit ear. Movat's Pentachrome. | Balloon angioplasty with restenosis injury rabbit ear. Movat's Pentachrome. |
Models of Atherosclerosis
Progression and regression studies in ApoE mice
ApoE mouse, high fat diet. The aorta is occluded with cholesterol plaques. Movat's pentachrome.
Feeding a high fat diet to ApoE mice as well as rabbits, guinea pigs and swine result in hyperlipidemia, steatosis and atherosclerotic arterial changes.