CBI offers a wide variety of validated models for the assessment of allergic and immune mediated disease including arthritis, EAE, colitis, dermatitis, eosinophilic esophagitis, and asthma. For more information, study designs and validation data, contact us at CBI and we will be happy to provide this information.
Arthritis
CBI has extensive experience in assessment of arthritis in mice, rats, and rabbits, including collagen-induced arthritis in DBA-1 mice, antibody-induced arthritis in mice, collagen and adjuvant-induced arthritis in rats, ovalbumin-induced arthritis in rabbits and MSU-induced models of gout. In vivo assessments may include clinical signs, biomarkers, body weights, food consumption, lameness scoring, joint diameter measurement scoring, plesthysmometry, and biomarkers. Histopathologic assessment including special stains and immunohistochemistry are key factors in assessing changes in the joints. Validated arthritis models at CBI include:
- Collagen-induced arthritis in mice
- Antibody-induced arthritis in mice
- Collagen-induced arthritis in rat
- Adjuvant-induced arthritis in rats
- Ovalbumin-induced arthritis in rabbits
- MSU-induced arthritis in mice (gout, and included intraperitoneal and air pouch inflammation)
For more information and validation data, contact CBI
Experimental Allergic Encephalomyelitis
CBI has extensive experience in assessment of Experimental Allergic Encephalomyelitis (EAE) as a model of MS in humans. Progressive or relapsing remitting forms in both mice and rats may be induced using MOG and PLP respectively. In vivo may include body weights, food consumption, neurologic scoring and biomarkers. Histopathologic assessment including special stains and immunohistochemistry are key factors in assessing test article activity on brain and spinal cord lesions.
For more information and validation data, contact CBI
Eosinophilic esophagitis
Eosinophilic esophagitis (EE) is an immune-mediated inflammatory condition in children and adults; its cause is not understood. CBI offers a mouse model of EE and pneumonitis induced by sensitization to Aspergillus fumigatus antigen. In vivo assessments may include body weights, clinical signs and serum biomarkers. Histopathologic assessment including special stains and immunohistochemistry are key factors in assessing test article activity in the esophagus and lungs.
For more information and validation data, contact CBI
Dermal delayed-type hypersensitivity
Dermal inflammation maybe induced by sensitization and challenge with haptens such as oxalazone or DNTB on the mouse ear or skin. CBI offers a robust mouse model of DTH. In vivo assessments may include ear thickness measurements, body weights, clinical signs and serum biomarkers. Histopathologic assessment including special stains and immunhistochemistry are key factors in assessing test article activity in the esophagus and lungs.
For more information and validation data, contact CBI
Colitis
CBI has extensive experience in assessment of colitis as a model of inflammatory bowel disease in mice and rats, including DSS, TNBS, and oxalazone-induced methods. In vivo assessments may include clinical observations, body weights, food consumption, and biomarker assays. Histopathologic assessment and colon weights are a key factor in assessing test article activity on the colonic mucosa. Histopathologic assessment including special stains and immunhistochemistry are key factors in assessing test article activity in the colon.
For more information and validation data, contact CBI
Asthma in Guinea pigs;
For more information and validation data, contact CBI
For information on DTH, Guinea Pig Sensitization, and other dermal immune mediated studies, see our information on Dermal Pharmacology.