Laser-Induced Macular Degeneration in Rats, Mice, and Rabbits
Laser-induced retinal neovascularization (macular degeneration LIMD) in mice, rats, and rabbits. In this model, subretinal lesions are induced in rats, mice, or rabbits using an surgical argon laser. Treatment is administered and plaque size is determined at the end of the study.
A typical study design:
- Five groups with 10 animals per group recommended
- Fundus evaluation and photography using fluorescein dye
- Test article administration either intraocularly or systemically
- Histopathologic assessment and measuring of subretinal plaque size
Retinopathy of prematurity model in infant mice and rats
In this animal model of oxygen-induced retinopathy (ROP), rat or mouse pups are placed in a hyperoxygenated enviroment for 2 weeks. Following a period of re-acclimation to normal air and treatment, the retinas are assessed for neovascularization.
A typical study design:
- 5 Groups with at least 20 pups per group recommended
- Test article administration either intraocularly or systemically
- FITC treated retinal whole mounts examined.
- Histopathologic assessment and measuring of subretinal plaque size
Corneal pockets in rats and rabbits for neovascularization
In this model of angiogenesis, a micropocket is surgically created and the test article is placed in the pocket. Neovascularization is measured. VEGF is the positive control.
A typical study design:
- Five groups with at least eight per group recommended (rats or rabbits)
- Test article administration topically or systemically
- Histopathologic assessment and measuring of subretinal plaque size